Publications

Chan KY, Alonso-Nuñez ML, Grallert A, Tanaka K, Connolly Y, Smith DL, Hagan IM. Dialogue between centrosomal entrance and exit scaffold pathways regulates mitotic commitment. Journal of Cell Biology pii: jcb.201702172. doi: 10.1083/jcb.201702172. [Epub ahead of print] (2017)

Grallert A§, Boke E§, Hagting A, Hodgson B, Connolly Y, Griffiths JR, Smith DL, Pines J, Hagan IM.  A PP1-PP2A phosphatase relay controls mitotic progression.  Nature 517:94-98 doi:10.1038/nature14019 (2015) §Equal contribution

Carpy A.§, Patel A.§, Tay YD, Hagan IM, Maček B. Nic1 Inactivation Enables Stable Isotope Labeling with 13C615N4-Arginine in Schizosaccharomyces pombe. Molecular and Cellular Proteomics. 14:243-50 (2015) §Equal contribution

Fu, J, Hagan, IM, Glover, DM  The centrosome and its duplication cycle. Cold Spring Harb Perspect Biol 7:pii: a015800. doi: 10.1101/cshperspect.a015800. (2015)

Fennessy D, Grallert A, Krapp A, Cokoja A, Bridge AJ, Petersen J, Patel A, Tallada VA, Boke E, Hodgson B, Simanis V, Hagan IM. Extending the Schizosaccharomyces pombe molecular genetic toolbox. PLoS One. 9(5):e97683. (2014)

Hagan IM, Grallert A. Spatial control of mitotic commitment in fission yeast. Biochem Soc Trans. 41: 1766-1771. (2013)

Tay YD, Patel A, Kaemena DF, Hagan IM.  Mutation of a conserved residue enhances sensitivity of analogue sensitized kinases to generate a novel approach for mitotic studies in fission yeast. Journal of Cell Science.126: 5052-5061. (2013)

Grallert A§, Chan KY§, Alonso-Nuñez ML, Madrid M, Biswas A, Alvarez-Tabarés I, Connolly Y, Tanaka K, Robertson A, Ortiz J-M, Smith DL, Hagan IM.  Removal of centrosomal PP1 by NIMA Kinase unlocks the MPF feedback loop to promote mitotic commitment in S. pombe. Current Biology.  23:213-222. (2013) §Equal contribution

Grallert A§, Patel A§, Tallada VA,Chan KY, Bagley S, Krapp A, Simanis V, Hagan IM.  Centrosomal MPF triggers the mitotic and morphogenetic switches of fission yeast. Nature Cell Biology. 15:88-95. (2013) §Equal contribution

Grallert, A., Connolly, Y., Smith, D. L., Simanis, V., Hagan, I.M. The S. pombe cytokinesis NDR kinase Sid2 activates Fin1 NIMA kinase to control mitotic commitment through Pom1/Wee1. Nature Cell Biology. 14: 738-745. (2012)

Funaya C, Samarasinghe S, Pruggnaller S, Ohta M, Connolly Y, Müller J, Murakami H, Grallert A, Yamamoto M, Smith D, Antony C, Tanaka K. Transient structure associated with the spindle pole body directs meiotic microtubule reorganization in S. pombe. Current Biology. 2012 22: 562-74. (2012)

Bitton DA§, Grallert A§, Scutt PJ, Yates T, Bradford JR, Li Y, Hey Y, Pepper SD, Hagan IM*, Miller CJ.* Programmed fluctuations in sense/anti-sense transcript ratios drive sexual differentiation in S. pombe. Molecular Systems Biology. 7:559. (2011) §Equal contribution *Corresponding authors

Pines J, Hagan IM. The Renaissance or the Cuckoo Clock. Philosophical Transaction of the Royal Society B.  366: 3625-3634. (2011)

Tamm T§, Grallert A§, Grossman EPS, Alvarez-Tabares I, Stevens FE, Hagan IM. Brr6 drives the S. pombe spindle pole body nuclear envelope insertion/extrusion cycle. Journal of Cell Biology. 195:467-484. (2011) §Equal contribution

Bitton DA, Wood V, Scutt PJ,   Grallert A, Yates T, Smith DL, Hagan IM, Miller CJ. Augmented Annotation of the Schizosaccharomyces pombe Genome Reveals Additional Genes Required for Growth and Viability. Genetics. 187: 1207- 1217. (2011)

Tallada, VA, Tanaka, K, Yanagida, M, Hagan, IM. The S. pombe mitotic regulator Cut12 promotes spindle pole body activation and integration into the nuclear envelope. Journal of Cell Biology. 185:875-88. (2009)

Robertson AM, Hagan IM. Stress-regulated kinase pathways in the recovery of tip growth and microtubule dynamics following osmotic stress in S. pombe. Journal of Cell Science. 121: 4055-4068. (2008)

Grallert A, Martín-García R, Bagley S, Mulvihill DP. in vivo movement of the type V myosin Myo52 requires dimerisation but is independent of the neck domain. Journal of Cell Science. 120:4093-4098. (2007)

Alvarez-Tabares I, Grallert A, Ortiz JM and Hagan IM. Schizosaccharomyces pombe protein phosphatase 1 in mitosis, endocytosis and a partnership with Wsh3/Tea4 to control polarised growth. Journal of Cell Science. 120: 3589-3601. (2007)

Tallada VA, Bridge AJ, Emery PE, Hagan IM. Suppression of the S. pombe cut12.1 cell cycle defect by mutations in cdc25 and genes involved in transcriptional and translational control. Genetics. 176:73-83. (2007)

Grallert A, Beuter C, Craven RA, Bagley S, Wilks D, Fleig U, Hagan IM. S. pombe CLASP needs dynein, not EB1 or CLIP170, to induce microtubule instability and slows polymerization rates at cell tips in a dynein-dependent manner. Genes and Development. 20:2421-36. (2006)

Petersen J, Hagan IM.  Polo kinase links the stress pathway to cell cycle control and tip growth in fission yeast. Nature. 435:507-12. (2005)

Unwin RD, Griffiths JR, Leverentz MK, Grallert A, Hagan IM, Whetton AD. Multiple reaction monitoring to identify sites of protein phosphorylation with high sensitivity. Molecular and Cellular Proteomics. 4:1134-1144. (2005)

Grallert A, Krapp A, Bagley S, Simanis V, Hagan IM. Recruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity. Genes and Development. 18: 1007-1021.(2004)

MacIver FH, Glover DM, Hagan IM. A “marker switch” approach to introduce mutated genes at endogenous loci in Schizosaccharomyces pombe. Yeast. 20:587-594. (2003)

Petersen J, Hagan IM. S. pombe aurora kinase/survivin is required for chromosome condensation and the spindle checkpoint attachment response. Current Biology. 13 590-597 (2003)

MacIver FH, Tanaka K, Robertson AM, Hagan IM. Physical and functional interactions between polo kinase and the spindle pole component Cut12 regulate mitotic commitment in S. pombe. Genes and Development. 17 1507-1523. (2003)

Grallert A, Hagan IM. S. pombe NIMA related kinase, Fin1, regulates spindle formation, and an affinity of Polo for the SPB. EMBO Journal 21:3096-107. (2002)

Anderson M, Ng SS, Marchesi V, MacIver FH, Stevens F E, Riddell T, Glover DM, Hagan IM, McInerny CJ. plo1+ regulates gene transcription at the M-G1 interval during the fission yeast mitotic cell cycle. EMBO Journal 21:5745-55. (2002)

Petersen J, Paris J, Willer M, Philippe M and Hagan IM. (2001). The S. pombe aurora-related kinase Ark1 asscoiates with mitotic structures in a stage dependent manner and is required for chromosome segregation. Journal of Cell Science 114:4371-4385. (2001)

Heitz MJ, Petersen J, Hagan IM. MTOC formation during mitotic exit in fission yeast. Journal of Cell Science 114: 4521-4533. (2001)

Cullen F, May K, Hagan IM, Glover DM, Ohkura H. A new genetic method to isolate functionally interacting genes – high plo1+ dependent mutants and their suppressors define genes in mitotic and septation pathways in fission yeast. Genetics. 155 1521-1534. (2000)

Publications Pre 2000

Mulvihill D P, Petersen J, Ohkura H, Glover DM, Hagan IM. Plo1 kinase recruitment to the spindle pole body and its role in cell division in Schizosaccharomyces pombe. Molecular Biology of the Cell. 10 2771--2785. (1999)

Mundt KE, Porte J, Murray JM, Brikos C, Christensen PU, Caspari T, Hagan IM, Millar JBA, Simanis V, Hoffman K, Carr AM. The Cop9/signalosome complex is conserved in fission yeast and has a role in S phase. Current Biology. 9 1427-1430. (1999)

Sohrmann M, Schmidt S, Hagan IM, Simanis V. Asymmetric, GTPase mediated, segregation on the spindle poles of the Schizosaccharomyces pombe septum-inducing protein kinase Cdc7p. Genes and Development. 12: 84-94. (1998)

Drummond DR, Hagan IM. Mutations in the bimC box of Cut7 indicate divergence of regulation within the bimC family of kinesin related proteins. Journal of Cell Science. 111: 853 867. (1998)

Petersen J, Nielsen O, Egel R, Hagan IM. F-actin function and localisation during the sexual differentiation of Schizosaccharomyces pombe. Journal of Cell Science. 111:867-876. (1998)

Bridge AJ, Morphew M, Bartlett R, Hagan IM. The fission yeast SPB component Cut12 links bipolar spindle formation to mitotic control. Genes and Development. 12:914-926. (1998)

Petersen J, Nielsen O, Egel R, Hagan IM. FH3, a previously unidentified domain shared by all formins targets fission yeast Fus1 to the projection tip during conjugation. Journal of Cell Biology. 141: 1207 - 1215. (1998)

Petersen J, Heitz MJ, Hagan IM. Conjugation in S. pombe: identification of a microtubule organising centre, a requirement for microtubules and a role for Mad2. Current Biology. 8:963-966. (1998)

Craven RA, Griffiths DJF, Sheldrick KS, Randal RE, Hagan IM. and Carr, A. M. Vectors for the expression of tagged proteins in Schizosaccharomyces pombe. Gene. 221:59-68. (1998)

Glover DM, Hagan IM, Tavares AAM. Polo kinases: a team that plays throughout mitosis. Genes and Development. 12: 3777-3787. (1998)

Hagan IM, Yanagida M. Evidence for cell cycle specific nuclear positioning in fission yeast. Journal of Cell Science. 110:1851-1866. (1997)

Beinhauer JD, Hagan IM, Hegemann JH, Fleig U. Mal3, the fission yeast homolog of the human APC-interacting protein EB-1 is required for microtubule integrity and the maintenance of Cell Form. Journal of Cell Biology. 139:717-728. (1997)

Hagan IM, Hyams JS. Forces acting on the fission yeast anaphase spindle. Cell Motility and the Cytoskeleton. 34:69-75. (1996)

Al-Khodairy F, Enoch T, Hagan IM, Carr AM. The S.pombe hus5+ gene encodes a ubiquitin conjugating enzyme required for normal mitosis. Journal of Cell Science. 108:475-486. (1995)

Hagan I, Yanagida M. The product of the spindle formation gene sad1+ associates with the fission yeast spindle pole body and is essential for viability. Journal of Cell Biology. 129:1033-1047. (1995)

Ohkura, H., Hagan , I. M. & Glover, D. M. The conserved Schizosaccharomyces pombe kinase Plo1, required to form a bipolar spindle, the actin ring, and septum, can drive septum formation in G1 and G2 cells. Genes and Development 9:1059 - 1073 (1995)

Carr. A.M., Moudjou, M., Bentley, N.J. & Hagan I. The chk1 pathway is required to prevent mitosis following cell cycle arrest at 'start'. Current Biology 5 1179-1190 (1995)

Funabiki, H, Hagan I., Uzawa, S. & Yanagida, M. Cell-cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast. Journal of Cell Biology 121:961-976 (1993)

Hagan I, Yanagida M. Kinesin related cut7 protein associates with mitotic and meiotic spindles in fission yeast. Nature. 356 74-76. (1992)

Hagan I, Yanagida M. Novel potential mitotic motor protein encoded by the fission yeast cut7+ gene. Nature. 347 563-566. (1990)

Hagan IM, Riddle PN, Hyams JS. Intramitotic controls in fission yeast: The effect of cell size upon the length and timing of mitotic events. Journal of Cell Biology. 110 1617-1621. (1990)

Hagan IM, Hyams JS. The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe. Journal of Cell Science. 89 343-357. (1988)

Hagan I, Hayles J, Nurse P. Cloning and sequencing of the cyclin related cdc13+ gene and a cytological investigation of its role in fission yeast mitosis. Journal of Cell Science.  91 587-595. (1988)

Marks J, Hagan IM, Hyams JS. Spatial association of F-actin with growth polarity in fission yeast. S.G.M. Symposium. 23 119-135. (1987)

Marks J, Hagan IM, Hyams JS. Growth polarity and cytokinesis in fission yeast, the role of the cytoskeleton. Journal of Cell Science Suppl. 5 229-241. (1986)