Reprogramming

 

 

Direct lineage reprogramming provides an alternative tool to ES and iPS cells for regenerative medicine. Recent reports have shown that somatic cells, under appropriate culture conditions, could be directly reprogrammed to cardiac, hepatic or neuronal phenotype by lineage specific transcription factors.   Work from our laboratory demonstrate that murine embryonic fibroblasts as well as adult fibroblasts can be directly reprogrammed to blood cells by over expression of hematopoietic transcription factors. Cell surface marker, transcriptome and cytospin analyses on reprogrammed cells established their hematopoietic nature. Reprogrammed cells can be differentiated to erythroid, myeloid and to some extent lymphoid lineages under specific culture conditions. Our results also suggest that fibroblasts upon TFs inductions are reprogrammed to hematopoietic cells via a progenitor state called hemogenic endothelial but not pluripotent stage. Importantly, as in the case of reprogramming to dedifferentiated stage, loss of p53 function facilitates reprograming to blood. Taken together our data provide an impetus to further evaluate direct reprogramming as an alternate approach to generate blood cell populations for autologous cell replacement therapies.