The Molecular Biology Core Facility

Automated Library Preparation for Next Generation Sequencing

The Molecular Biology Core Facility provides a wide array of services for high precision, high throughput analysis of DNA and RNA to support molecular biology projects within the CRUK Manchester Institute.

 

 

 

 

We offer comprehensive services for Sanger Sequencing and Next Generation Sequencing. Employing three Illumina platforms we cover a wide range of NGS applications including

Whole Genome and Whole Exome Sequencing as well as RNAseq, ChIPseq and Targeted Sequencing.

In addition, MBCF hosts a range of state-of–the-art instruments and offers technical expertise for genome and gene expression analysis, including high-throughput Real-time Quantitative PCR, as well as Digital PCR. We are also set up for single cell isolation (Fluidigm C1) for genome and transcriptome analysis.

Registered users of the MBCF please use the PPMS calendar system for booking equipment time and services.

For further information about any of the technologies or applications within the MBCF please email  Wolfgang Breitwieser

Technology Platforms:


Sanger Sequencing:          ABI Prism 3130xl Genetic Analyzer

Clonal Sequencing:          Illumina Hiseq2500

                                              Illumina Nextseq500

                                              Illumina Miseq

 

Sample QC:                        Agilent 2100 Bioanalyzer

                                              Agilent 2200 TapeStation

 

QPCR:                                   Applied Biosystems Quantstudio 3

                                               Applied Biosystems Quantstudio 5

                                               Fluidigm Biomark HD

 

Digital PCR:                         BioRad QX200 Droplet Digital PCR

                                                         incl. Auto Droplet Generator

                                                         QX200 Droplet Reader

                                                         T100 Thermal Cycler


Single Cell Prep System:           Fluidigm C1

 

High Throughput Compound Handling:                 Eppendorf epMotion 5070

                                                                                        Agilent Bravo

                                                                                        Formulatrix Mantis

                                                                                        Labcyte Access/Echo550

Infrared Imaging:                 Li-Cor Odyssey CLx

Array Scanner:                      Molecular Devices GenePix 4100A

 

 Sonication:                            Diagenode Bioruptor Pico