The Molecular Biology Core Facility
Automated Library Preparation for Next Generation Sequencing
The Molecular Biology Core Facility provides a wide array of services for high precision, high throughput analysis of DNA and RNA to support molecular biology projects within the CRUK Manchester Institute.
We offer comprehensive services for Sanger Sequencing and Next Generation Sequencing. Employing three Illumina platforms we cover a wide range of NGS applications including
Whole Genome and Whole Exome Sequencing as well as RNAseq, ChIPseq and Targeted Sequencing.
In addition, MBCF hosts a range of state-of–the-art instruments and offers technical expertise for genome and gene expression analysis, including high-throughput Real-time Quantitative PCR, as well as Digital PCR. We are also set up for single cell isolation (Fluidigm C1) for genome and transcriptome analysis.
Registered users of the MBCF please use the PPMS calendar system for booking equipment time and services.
For further information about any of the technologies or applications within the MBCF please email Wolfgang Breitwieser.
Sanger Sequencing: ABI Prism 3130xl Genetic Analyzer
Clonal Sequencing: Illumina Hiseq2500
Pyrosequencing: Qiagen Pyromark Q24
Sample QC: Agilent 2100 Bioanalyzer
Agilent 2200 TapeStation
QPCR: Applied Biosystems Quantstudio 3
Applied Biosystems Quantstudio 5 Fluidigm Biomark HD
Digital PCR: BioRad QX200 Droplet Digital PCR
incl. Auto Droplet Generator
QX200 Droplet Reader
T100 Thermal Cycler
Single Cell Prep System: Fluidigm C1
High Throughput Compound Handling: Eppendorf epMotion 5070
Infrared Imaging: Li-Cor Odyssey CLx
Array Scanner: Molecular Devices GenePix 4100A
Sonication: Diagenode Bioruptor Pico