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WORLD CLASS BASIC, TRANSLATIONAL AND CLINICAL RESEARCH

Techniques for Transgenic Mouse Production

Pronuclear Microinjection Platform

  • Project design and discussion
  • Generation of the targeting construct (optional)
  • Injection of DNA into zygotes for production of founder mice
  • Genotyping to identify F0 founders
  • Mating of F0s to obtain germline-confirmed F1 mice

ES cell targeting Platform

  • Project design and discussion
  • Generation of targeting construct (optional)
  • ES cell targeting and selection
  • Screening and expansion of positive clones
  • Microinjection of 2-3 positive clones into host blastocysts
  • Chimera genotyping and breeding

CRISPR-Cas Platform

  • Project strategy design and discussion
  • Preparation of reagents based on specific project requirements
  • Injection of CRISPR reagents into zygotes for production of founder mice
  • Genotyping to identify F0 founders
  • Mating of F0s to obtain germline-confirmed F1 mice