Recent Publications

Biological Mass Spectrometry Facility

Mitochondria as new therapeutic targets for eradicating cancer stem cells: Quantitative proteomics and functional validation via MCT1/2 inhibition.
Lamb R, Harrison H, Hulit J, Smith DL, Lisanti MP, Sotgia F.
Oncotarget. 2014 Nov 15. [Epub ahead of print]

Cleavage by signal peptide peptidase is required for the degradation of selected tail-anchored proteins.
Boname JM, Bloor S, Wandel MP, Nathan JA, Antrobus R, Dingwell KS, Thurston TL, Smith DL, Smith JC, Randow F, Lehner PJ.
J Cell Biol. 2014 Jun 23;205(6):847-62. doi: 10.1083/jcb.201312009.

Mass Spectral Enhanced Detection of Ubls Using SWATH Acquisition: MEDUSA—Simultaneous Quantification of SUMO and Ubiquitin-Derived Isopeptides
John R Griffiths, Navin Chicooree, Yvonne Connolly, Milla Neffling, Catherine S Lane, Thomas Knapman & Duncan L Smith
J.Am.Soc. Mass Spectrom. 2014 May;25(5):767-77. doi: 10.1007/s13361-014-0835-x. Epub 2014 Feb 19.

Chemically facilitating the generation of diagnostic ions from SUMO(2/3) remnant isopeptides
Chicooree N, Griffiths JR, Connolly Y,  Smith DL.
Rapid Commun Mass Spectrom. 2013 Sep 30; 27(18):2108-14.

Latency-associated degradation of the MRP1 drug transporter during latent human cytomegalovirus infection.
Weekes MP, Tan SY, Poole E, Talbot S, Antrobus R, Smith DL, Montag C, Gygi SP, Sinclair JH, Lehner PJ.
Science. 2013 Apr 12;340(6129):199-202. doi: 10.1126/science.

Application of the CIRAD mass spectrometry approach for lysine acetylation site discovery.
Evans CA, Ow SY, Smith DL, Corfe BM, Wright PC.
Methods Mol Biol. 2013;981:13-23. doi: 10.1007/978-1-62703-305-3_2.

Enhanced detection of ubiquitin isopeptides using reductive methylation.
Chicooree N, Connolly Y, Tan CT, Malliri A, Li Y, Smith DL, Griffiths JR.
J Am Soc Mass Spectrom. 2013 Mar;24(3):421-30. doi: 10.1007/s13361-012-0538-0. Epub 2013 Jan 30.

Removal of centrosomal PP1 by NIMA kinase unlocks the MPF feedback loop to promote mitotic commitment in S. pombe.
Grallert A, Chan KY, Alonso-Nuñez ML, Madrid M, Biswas A, Alvarez-Tabarés I, Connolly Y, Tanaka K, Robertson A, Ortiz JM, Smith DL, Hagan IM.
Curr Biol. 2013 Feb 4;23(3):213-22. doi: 10.1016/j.cub.2012.12.039. Epub 2013 Jan 17.

A novel approach to the analysis of SUMOylation with the independent use of trypsin and elastase digestion followed by database searching utilising consecutive residue addition to lysine.
Chicooree N, Griffiths JR, Connolly Y, Tan CT, Malliri A, Eyers CE, Smith DL.
Rapid Commun Mass Spectrom. 2013 Jan 15;27(1):127-34. doi: 10.1002/rcm.6425.

Cell Division

A PP1-PP2A phosphatase relay controls mitotic progression.
Grallert A, Boke E, Hagting A, Hodgson B, Connolly Y, Griffiths JR, Smith DL, Pines J, Hagan IM.
Nature. 2014 Dec 10. doi: 10.1038/nature14019. [Epub ahead of print]

Extending the Schizosaccharomyces pombe molecular genetic toolbox.
Fennessy D, Grallert A, Krapp A, Cokoja A, Bridge AJ, Petersen J, Patel A, Tallada VA, Boke E, Hodgson B, Simanis V, Hagan IM.
PLoS One. 2014 May 21;9(5):e97683

 

Mutation of a conserved residue enhances the sensitivity of analogue-sensitised kinases to generate a novel approach to the study of mitosis in fission yeast.
Tay YD, Patel A, Kaemena DF, Hagan IM.
J Cell Sci. 2013 Nov 1;126(Pt 21):5052-61. doi: 10.1242/jcs.135301. Epub 2013 Aug 28.

Removal of Centrosomal PP1 by NIMA Kinase Unlocks the MPF Feedback Loop to Promote Mitotic Commitment in S. pombe.
Grallert A, Chan KY, Alonso-Nuñez ML, Madrid M, Biswas A, Alvarez-Tabarés I, Connolly Y, Tanaka K, Robertson A, Ortiz JM, Smith DL, Hagan IM.
Curr Biol. 2013 Feb 4;23(3):213-22. doi: 10.1016/j.cub.2012.12.039. Epub 2013 Jan 17. PMID: 23333317 [PubMed - in process]

Cell Regulation

 

JNK Suppresses Tumor Formation via a Gene-Expression Program Mediated by ATF2.
Gozdecka M, Lyons S, Kondo S, Taylor J, Li Y, Walczynski J, Thiel G, Breitwieser W, Jones N.
Cell Rep. 2014 Nov 20;9(4):1361-74. doi: 10.1016/j.celrep.2014.10.043. Epub 2014 Nov 13.

 

Sensitisation of c-MYC-induced B-lymphoma cells to apoptosis by ATF2.
Walczynski J, Lyons S, Jones N, Breitwieser W.
Oncogene. 2013 Feb 18. doi: 10.1038/onc.2013.28. [Epub ahead of print] PMID: 23416976 [PubMed - as supplied by publisher]

Cell Signalling

HUWE1 Ubiquitylates and Degrades the RAC Activator TIAM1 Promoting Cell-Cell Adhesion Disassembly, Migration, and Invasion.
Vaughan L, Tan C, Chapman A, Nonaka D, Mack NA, Smith D, Booton R, Hurlstone AF, Malliri A.
Cell Rep. 2014 Dec 23. pii: S2211-1247(14)01041-9. doi: 10.1016/j.celrep.2014.12.012. [Epub ahead of print]

Hace1 controls ROS generation of vertebrate Rac1-dependent NADPH oxidase complexes.
Daugaard M, Nitsch R, Razaghi B, McDonald L, Jarrar A, Torrino S, Castillo-Lluva S, Rotblat B, Li L, Malliri A, Lemichez E, Mettouchi A, Berman JN, Penninger JM, Sorensen PH.
Nat Commun. 2013 Jul 17;4:2180. doi: 10.1038/ncomms3180.

 

Enhanced detection of ubiquitin isopeptides using reductive methylation.
Chicooree N, Connolly Y, Tan CT, Malliri A, Li Y, Smith DL, Griffiths JR.
J Am Soc Mass Spectrom. 2013 Mar;24(3):421-30. doi: 10.1007/s13361-012-0538-0. Epub 2013 Jan 30.

Constitutive Rac Activation Is Not Sufficient to Initiate Melanocyte Neoplasia but Accelerates Malignant Progression.
Dalton LE, Kamarashev J, Barinaga-Rementeria Ramirez I, White G, Malliri A, Hurlstone A.
J Invest Dermatol. 2013 Jan 21. doi: 10.1038/jid.2013.23. [Epub ahead of print] PMID: 23337888 [PubMed - as supplied by publisher]

 

Clinical and Experimental Pharmacology

Tracking genomic cancer evolution for precision medicine: the lung TRACERx study.
Jamal-Hanjani M, Hackshaw A, Ngai Y, Shaw J, Dive C, Quezada S, Middleton G, de Bruin E, Le Quesne J, Shafi S, Falzon M, Horswell S, Blackhall F, Khan I, Janes S, Nicolson M, Lawrence D, Forster M, Fennell D, Lee SM, Lester J, Kerr K, Muller S, Iles N, Smith S, Murugaesu N, Mitter R, Salm M, Stuart A, Matthews N, Adams H, Ahmad T, Attanoos R, Bennett J, Birkbak NJ, Booton R, Brady G, Buchan K, Capitano A, Chetty M, Cobbold M, Crosbie P, Davies H, Denison A, Djearman M, Goldman J, Haswell T, Joseph L, Kornaszewska M, Krebs M, Langman G, MacKenzie M, Millar J, Morgan B, Naidu B, Nonaka D, Peggs K, Pritchard C, Remmen H, Rowan A, Shah R, Smith E, Summers Y, Taylor M, Veeriah S, Waller D, Wilcox B, Wilcox M, Woolhouse I, McGranahan N, Swanton C.
PLoS Biol. 2014 12(7):e1001906.

The combination of circulating ang1 and tie2 levels predicts progression-free survival advantage in bevacizumab-treated patients with ovarian cancer.
Backen A, Renehan AG, Clamp AR, Berzuini C, Zhou C, Oza A, Bannoo S, Scherer SJ, Banks RE, Dive C, Jayson GC.
Clin Cancer Res. 2014 20(17):4549-58.

Circulating biomarkers in hepatocellular carcinoma.
Morris KL, Tugwood JD, Khoja L, Lancashire M, Sloane R, Burt D, Shenjere P, Zhou C, Hodgson C, Ohtomo T, Katoh A, Ishiguro T, Valle JW, Dive C.
Cancer Chemother Pharmacol. 2014 74(2):323-32

Tumorigenicity and genetic profiling of circulating tumor cells in small-cell lung cancer.
Hodgkinson CL, Morrow CJ, Li Y, Metcalf RL, Rothwell DG, Trapani F, Polanski R, Burt DJ, Simpson KL, Morris K, Pepper SD, Nonaka D, Greystoke A, Kelly P, Bola B, Krebs MG, Antonello J, Ayub M, Faulkner S, Priest L, Carter L, Tate C, Miller CJ, Blackhall F, Brady G, Dive C.
Nat Med. 2014 20(8):897-903.

Quantification of skeletal metastases in castrate-resistant prostate cancer predicts progression-free and overall survival.
Tait C, Moore D, Hodgson C, Brown M, Morris T, Growcott J, Malone M, Hughes A, Renehan A, Clarke NW, Dive C.
BJU Int. 2014 Mar 3. doi: 10.1111/bju.12717. [Epub ahead of print]

Molecular analysis of circulating tumour cells-biology and biomarkers.
Krebs MG, Metcalf RL, Carter L, Brady G, Blackhall FH, Dive C.
Nat Rev Clin Oncol. 2014 11(3):129-44.

Prevalence and heterogeneity of circulating tumour cells in metastatic cutaneous melanoma.
Khoja L, Shenjere P, Hodgson C, Hodgetts J, Clack G, Hughes A, Lorigan P, Dive C.
Melanoma Res. 2014 24(1):40-6.

Assessment of diurnal changes and confounding factors that affect circulating cell death biomarker levels: a short communication.
Greystoke A, Harris G, Jenkins M, Goonetilleke D, Moore D, Lancashire M, Ranson M, Hughes A, Clack G, Dive C.
J Pharm Biomed Anal. 2013 Oct;84:184-8. doi: 10.1016/j.jpba.2013.06.010. Epub 2013 Jun 21.

[18F]-FLT positron emission tomography can be used to image the response of sensitive tumors to PI3-kinase inhibition with the novel agent GDC-0941.
Cawthorne C, Burrows N, Gieling RG, Morrow CJ, Forster D, Gregory J, Radigois M, Smigova A, Babur M, Simpson K, Hodgkinson C, Brown G, McMahon A, Dive C, Hiscock D, Wilson I, Williams KJ.
Mol Cancer Ther. 2013 May;12(5):819-28. doi: 10.1158/1535-7163.MCT-12-0905. Epub 2013 Feb 20.

Biomarker utility of circulating tumor cells in metastatic cutaneous melanoma.
Khoja L, Lorigan P, Zhou C, Lancashire M, Booth J, Cummings J, Califano R, Clack G, Hughes A, Dive C.
J Invest Dermatol. 2013 Jun;133(6):1582-90. doi: 10.1038/jid.2012.468. Epub 2012 Dec 6.

Drug Discovery

Reversible Inhibitors of LSD1 as Therapeutic Agents in Acute Myeloid Leukemia: Clinical Significance and Progress to Date.
Mould DP, McGonagle AE, Wiseman DH, Williams EL, Jordan AM.
Med Res Rev. 2014 Nov 24. doi: 10.1002/med.21334. [Epub ahead of print]

Elevated plasma 2-hydroxyglutarate in acute myeloid leukaemia: association with the IDH1 SNP rs11554137 and severe renal impairment.
Wiseman DH, Small HF, Wilks DP, Waddell ID, Dennis MW, Ogilvie DJ, Somervaille TC.
Br J Haematol. 2014 Jul;166(1):145-8.

Toxoflavins and Deazaflavins as the First Reported Selective Small Molecule Inhibitors of Tyrosyl-DNA Phosphodiesterase II.
Raoof A, Depledge P, Hamilton NM, Hamilton NS, Hitchin JR, Hopkins GV, Jordan AM, Maguire LA, McGonagle AE, Mould DP, Rushbrooke M, Small HF, Smith KM, Thomson GJ, Turlais F, Waddell ID, Waszkowycz B, Watson AJ, Ogilvie DJ.
J Med Chem. 2013 Jul 31. [Epub ahead of print]

A fluorescence-based assay for the apurinic/apyrimidinic-site cleavage activity of human tyrosyl-DNA phosphodiesterase 1.
Thomson GJ, Hamilton NS, Hopkins GV, Waddell ID, Watson AJ, Ogilvie DJ.
Anal Biochem. 2013 Sep 1;440(1):1-5.

Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase.
Thomson G, Watson A, Caldecott K, Denneny O, Depledge P, Hamilton N, Hopkins G, Jordan A, Morrow C, Raoof A, Waddell I, Ogilvie D.
Anal Biochem. 2013 May 15;436(2):145-50.

Leukaemia Biology

 

Reversible Inhibitors of LSD1 as Therapeutic Agents in Acute Myeloid Leukemia: Clinical Significance and Progress to Date.
Mould DP, McGonagle AE, Wiseman DH, Williams EL, Jordan AM.
Med Res Rev. 2014 Nov 24. doi: 10.1002/med.21334. [Epub ahead of print]

 

Elevated plasma 2-hydroxyglutarate in acute myeloid leukaemia: association with the IDH1 SNP rs11554137 and severe renal impairment.
Wiseman DH, Small HF, Wilks DP, Waddell ID, Dennis MW, Ogilvie DJ, Somervaille TC.
Br J Haematol. 2014 Jul;166(1):145-8.

Enhancers of Polycomb EPC1 and EPC2 sustain the oncogenic potential of MLL leukemia stem cells.
Huang X, Spencer GJ, Lynch JT, Ciceri F, Somerville TD, Somervaille TC.
Leukemia. 2014 May;28(5):1081-91.

A targeted knockdown screen of genes coding for phosphoinositide modulators identifies PIP4K2A as required for acute myeloid leukemia cell proliferation and survival.
Jude JG, Spencer GJ, Huang X, Somerville TD, Jones DR, Divecha N, Somervaille TC.
Oncogene. 2014 Mar 31. doi: 10.1038/onc.2014.77. [Epub ahead of print]

Very high frequencies of leukaemia-initiating cells in precursor T-acute lymphoblastic leukaemia may be obscured by cryopreservation.
Greystoke BF, Huang X, Wilks DP, Atkinson S, Somervaille TC.
Br J Haematol. 2013 Nov; 163(4):538-41.

CD86 expression as a surrogate cellular biomarker for pharmacologic inhibition of the histone demethylase LSD1.
Lynch JT, Cockerill MJ, Hitchin JR, Wiseman DH, Somervaille TC.
Anal Biochem. 2013 Nov 1; 422(1):104-6.

Molecular Oncology

Paradox-Breaking RAF Inhibitors that Also Target SRC Are Effective in Drug-Resistant BRAF Mutant Melanoma.
Girotti
MR, Lopes F, Preece N, Niculescu-Duvaz D, Zambon A, Davies L, Whittaker S, Saturno G, Viros A, Pedersen M, Suijkerbuijk BM, Menard D, McLeary R, Johnson L, Fish L, Ejiama S, Sanchez-Laorden B, Hohloch J, Carragher N, Macleod K, Ashton G, Marusiak AA, Fusi A, Brognard J, Frame M, Lorigan P, Marais R, Springer C.
Cancer Cell. 2014 Dec 10. pii: S1535-6108(14)00457-7. doi: 10.1016/j.ccell.2014.11.006. [Epub ahead of print]

The Immune Microenvironment Confers Resistance to MAPK Pathway Inhibitors through Macrophage-Derived TNFα
Smith MP, Sanchez-Laorden B, O'Brien K, Brunton H, Ferguson J, Young H, Dhomen N, Flaherty KT, Frederick DT, Cooper ZA, Wargo JA, Marais R, Wellbrock C.
Cancer Discov. 2014 Sep 24. [Epub ahead of print]

 

G12D NRAS and kinase-dead BRAF cooperate to drive naevogenesis and melanomagenesis.
Pedersen M, Viros A, Cook M, Marais R.
Pigment Cell Melanoma Res. 2014 Jul 17. doi: 10.1111/pcmr.12293. [Epub ahead of print] No abstract available.

Ultraviolet radiation accelerates BRAF-driven melanomagenesis by targeting TP53.
Viros A, Sanchez-Laorden B, Pedersen M, Furney SJ, Rae J, Hogan K, Ejiama S, Girotti MR, Cook M, Dhomen N, Marais R.
Nature. 2014 Jul 24;511(7510):478-82.

The mutational burden of acral melanoma revealed by whole-genome sequencing and comparative analysis.
Furney SJ, Turajlic S, Stamp G, Thomas JM, Hayes A, Strauss D, Gavrielides M, Xing W, Gore M, Larkin J, Marais R.
Pigment Cell Melanoma Res. 2014 Sep;27(5):835-8.

BRAF inhibitors induce metastasis in RAS mutant or inhibitor-resistant melanoma cells by reactivating MEK and ERK signaling.
Sanchez-Laorden B, Viros A, Girotti MR, Pedersen M, Saturno G, Zambon A, Niculescu-Duvaz D, Turajlic S, Hayes A, Gore M, Larkin J, Lorigan P, Cook M, Springer C, Marais R.
Sci Signal. 2014 Mar 25;7(318):ra30.

Whole-genome sequencing reveals complex mechanisms of intrinsic resistance to BRAF inhibition.
Turajlic S, Furney SJ, Stamp G, Rana S, Ricken G, Oduko Y, Saturno G, Springer C, Hayes A, Gore M, Larkin J, Marais R.
Ann Oncol. 2014 May;25(5):959-67.

BRAF inhibitors induce metastasis in RAS mutant or inhibitor-resistant melanoma cells by reactivating MEK and ERK signaling.
Sanchez-Laorden B, Viros A, Girotti MR, Pedersen M, Saturno G, Zambon A, Niculescu-Duvaz D, Turajlic S, Hayes A, Gore M, Larkin J, Lorigan P, Cook M, Springer C, Marais R.
Sci Signal. 2014 Mar 25;7(318):ra30.

COX-2 inhibition prevents the appearance of cutaneous squamous cell carcinomas accelerated by BRAF inhibitors.
Escuin-Ordinas H, Atefi M, Fu Y, Cass A, Ng C, Huang RR, Yashar S, Comin-Anduix B, Avramis E, Cochran AJ, Marais R, Lo RS, Graeber TG, Herschman HR, Ribas A.
Mol Oncol. 2014 Mar;8(2):250-60.

A common alternative splicing signature is associated with SF3B1 mutations in malignancies from different cell lineages.
Gentien D, Kosmider O, Nguyen-Khac F, Albaud B, Rapinat A, Dumont AG, Damm F, Popova T, Marais R, Fontenay M, Roman-Roman S, Bernard OA, Stern MH.
Leukemia. 2014 Jan 17. doi: 10.1038/leu.2014.28. [Epub ahead of print]

SF3B1 mutations are associated with alternative splicing in uveal melanoma.
Furney SJ, Pedersen M, Gentien D, Dumont AG, Rapinat A, Desjardins L, Turajlic S, Piperno-Neumann S, de la Grange P, Roman-Roman S, Stern MH, Marais R.
Cancer Discov. 2013 Jul 16. [Epub ahead of print]

Genome sequencing of mucosal melanomas reveals that they are driven by distinct mechanisms from cutaneous melanoma.
Furney SJ, Turajlic S, Stamp G, Nohadani M, Carlisle A, Thomas JM, Hayes A, Strauss D, Gore M, van den Oord J, Larkin J, Marais R.
J Pathol. 2013 Jul;230(3):261-9.

Primary melanoma of the CNS in children is driven by congenital expression of oncogenic NRAS in melanocytes.
Pedersen M, Küsters-Vandevelde HV, Viros A, Groenen PJ, Sanchez-Laorden B, Gilhuis JH, van Engen-van Grunsven IA, Renier W, Schieving J, Niculescu-Duvaz I, Springer CJ, Küsters B, Wesseling P, Blokx WA, Marais R.
Cancer Discov. 2013 Apr;3(4):458-69.

Inhibiting EGF Receptor or SRC Family Kinase Signaling Overcomes BRAF Inhibitor Resistance in Melanoma.
Girotti MR, Pedersen M, Sanchez-Laorden B, Viros A, Turajlic S, Niculescu-Duvaz D, Zambon A, Sinclair J, Hayes A, Gore M, Lorigan P, Springer C, Larkin J, Jorgensen C, Marais R.
Cancer Discov. 2013 Feb;3(2):158-67. doi: 10.1158/2159-8290.CD-12-0386. Epub 2012 Dec 14. PMID: 23242808 [PubMed - in process]

Prostate Oncobiology

ETV1 directs androgen metabolism and confers aggressive prostate cancer in targeted mice and patients.
Baena E, Shao Z, Linn DE, Glass K, Hamblen MJ, Fujiwara Y, Kim J, Nguyen M, Zhang X, Godinho FJ, Bronson RT, Mucci LA, Loda M, Yuan GC, Orkin SH, Li Z.
Genes Dev. 2013 27(6):683-98.

RNA Biology

Cancer-Associated Protein Kinase C Mutations Reveal Kinase's Role as Tumor Suppressor
Antal CE, Hudson AM, Kang E, Zanca C, Wirth C, Stephenson NL, Trotter EW, Gallegos LL, Miller CJ, Furnari FB, Hunter T, Brognard J, Newton AC.
Cell. 2015 Jan 29;160(3):489-502.

Evaluation and validation of a robust single cell RNA-amplification protocol through transcriptional profiling of enriched lung cancer initiating cells
Dominic G Rothwell, Yaoyong Li, Mahmood Ayub, Catriona Tate, Gillian Newton, Yvonne Hey, Louise Carter, Suzanne Faulkner, Massimo Moro, Stuart Pepper, Crispin Miller, Fiona Blackhall, Giulia Bertolini, Luca Roz, Caroline Dive and Ged Brady
BMC Genomics 2014, 15:1129 doi:10.1186/1471-2164-15-1129

Discrepancies in Cancer Genomic Sequencing Highlight Opportunities for Driver Mutation Discovery
Hudson AM, Yates T, Li Y, Trotter EW, Fawdar S, Chapman P, Lorigan P, Biankin AV, Miller C,
Brognard J
Cancer Res; 2014 Sep 25. pii: canres.1020.2014. [Epub ahead of print]

Epithelial and Stromal MicroRNA Signatures of Columnar Cell Hyperplasia Linking Let-7c to Precancerous and Cancerous Breast Cancer Cell Proliferation.
Björner S, Fitzpatrick PA, Li Y, Allred C, Howell A, Ringberg A, Olsson H, Miller CJ, Axelson H, Landberg G.
PLoS One. 2014 Aug 14;9(8):e105099. doi:10.1371/journal.pone.0105099. eCollection 2014. 

RUNX1 positively regulates a cell adhesion and migration program in murine hemogenic endothelium prior to blood emergence.
Lie-A-Ling M, Marinopoulou E, Li Y, Patel R, Stefanska M, Bonifer C, Miller C, Kouskoff V, Lacaud G.
Blood. 2014 Sep 11;124(11):e11-20. doi: 10.1182/blood-2014-04-572958. Epub 2014 Jul 31.

Tumorigenicity and genetic profiling of circulating tumor cells in small-cell lung cancer.
Hodgkinson CL, Morrow CJ, Li Y, Metcalf RL, Rothwell DG, Trapani F, Polanski R, Burt DJ, Simpson KL, Morris K, Pepper SD, Nonaka D, Greystoke A, Kelly P, Bola B, Krebs MG, Antonello J, Ayub M, Faulkner S, Priest L, Carter L, Tate C, Miller CJ, Blackhall F, Brady G, Dive C.
Nat Med. 2014 20(8):897-903.

A global non-coding RNA system modulates fission yeast protein levels in response to stress.
Leong HS, Dawson K, Wirth C, Li Y, Connolly Y, Smith DL, Wilkinson CR, Miller CJ.
Nat Commun. 2014 May 23;5:3947. doi: 10.1038/ncomms4947.

Investigation of radiosensitivity gene signatures in cancer cell lines.
Hall JS, Iype R, Senra J, Taylor J, Armenoult L, Oguejiofor K, Li Y, Stratford I, Stern PL, O'Connor MJ, Miller CJ, West CM.
PLoS One. 2014 Jan 22;9(1):e86329.

Mzt1/Tam4, a fission yeast MOZART1 homologue, is an essential component of the γ-tubulin complex and directly interacts with GCP3Alp6.
Dhani DK, Goult BT, George GM, Rogerson DT, Bitton DA, Miller CJ, Schwabe JW, Tanaka K.
Mol Biol Cell. 2013 Nov;24(21):3337-49.

Targeted genetic dependency screen facilitates identification of actionable mutations in FGFR4, MAP3K9, and PAK5 in lung cancer.
Fawdar S, Trotter EW, Li Y, Stephenson NL, Hanke F, Marusiak AA, Edwards ZC, Ientile S, Waszkowycz B, Miller CJ, Brognard J.
Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12426-31.

Prospective technical validation and assessment of intra-tumour heterogeneity of a low density array hypoxia gene profile in head and neck squamous cell carcinoma.
Betts GN, Eustace A, Patiar S, Valentine HR, Irlam J, Ramachandran A, Merve A, Homer JJ, Möller-Levet C, Buffa FM, Hall G, Miller CJ, Harris AL, West CM.
Eur J Cancer. 2013 Jan;49(1):156-65.

Signalling Networks in Cancer

Cancer-Associated Protein Kinase C Mutations Reveal Kinase's Role as Tumor Suppressor.
Antal CE, Hudson AM, Kang E, Zanca C, Wirth C, Stephenson NL, Trotter EW, Gallegos LL, Miller CJ, Furnari FB, Hunter T, Brognard J*, Newton AC*.
Cell. 2015 Jan 29;160(3):489-502.   * Corresponding authors

Discrepancies in Cancer Genomic Sequencing Highlight Opportunities for Driver Mutation Discovery
Hudson AM, Yates T, Li Y, Trotter EW, Fawdar S, Chapman P, Lorigan P, Biankin AV, Miller C*, Brognard J*
Cancer Res; 2014 Sep 25. pii: canres.1020.2014. [Epub ahead of print]  * Corresponding authors

Mixed lineage kinases activate MEK independently of RAF to mediate resistance to RAF inhibitors.
Marusiak AA, Edwards ZC, Hugo W, Trotter EW, Girotti MR, Stephenson NL, Kong X, Gartside MG, Fawdar S, Hudson A, Breitwieser W, Hayward NK, Marais R, Lo RS, Brognard J.
Nat Commun. 2014 May 22;5:3901. doi: 10.1038/ncomms4901.

Targeted genetic dependency screen facilitates identification of actionable mutations in FGFR4, MAP3K9, and PAK5 in lung cancer.
Fawdar S, Trotter EW, Li Y, Stephenson NL, Hanke F, Marusiak AA, Edwards ZC, Ientile S, Waszkowycz B, Miller CJ, Brognard J.
Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12426-31.

Protein kinase cδ deficiency causes mendelian systemic lupus erythematosus with B cell-defective apoptosis and hyperproliferation.
Belot A, Kasher PR, Trotter EW, Foray AP, Debaud AL, Rice GI, Szynkiewicz M, Zabot MT, Rouvet I, Bhaskar SS, Daly SB, Dickerson JE, Mayer J, O'Sullivan J, Juillard L, Urquhart JE, Fawdar S, Marusiak AA, Stephenson N, Waszkowycz B, W Beresford M, Biesecker LG, C M Black G, René C, Eliaou JF, Fabien N, Ranchin B, Cochat P, Gaffney PM, Rozenberg F, Lebon P, Malcus C, Crow YJ, Brognard J, Bonnefoy N.
Arthritis Rheum. 2013 Aug;65(8):2161-71.

Stem Cell Biology

Direct reprogramming of murine fibroblasts to hematopoietic progenitor cells.
Batta K, Florkowska M, Kouskoff V, Lacaud G.
Cell Rep. 2014 Dec 11;9(5):1871-84.

 

RUNX1 positively regulates a cell adhesion and migration program in murine hemogenic endothelium prior to blood emergence.
Lie-A-Ling M, Marinopoulou E, Li Y, Patel R, Stefanska M, Bonifer C, Miller C, Kouskoff V, Lacaud G.
Blood. 2014 Sep 11;124(11):e11-20.

MOZ-mediated repression of p16(INK) (4) (a) is critical for the self-renewal of neural and hematopoietic stem cells.
Perez-Campo FM, Costa G, Lie-A-Ling M, Stifani S, Kouskoff V, Lacaud G.
Stem Cells. 2014 Jun;32(6):1591-601.

Smooth muscle cells largely develop independently of functional hemogenic endothelium.
Stefanska M, Costa G, Lie-A-Ling M, Kouskoff V, Lacaud G.
Stem Cell Res. 2014 Jan;12(1):222-32.

The MYSTerious MOZ, a Histone Acetyltransferase with a Key Role in Haematopoiesis.
Perez-Campo FM, Costa G, Lie-A-Ling M, Kouskoff V, Lacaud G.
Immunology. 2013 Jun;139(2):161-5.

 

Stem Cell Haematopoiesis

Direct reprogramming of murine fibroblasts to hematopoietic progenitor cells.
Batta K, Florkowska M, Kouskoff V, Lacaud G.
Cell Rep. 2014 Dec 11;9(5):1871-84.

RUNX1 positively regulates a cell adhesion and migration program in murine hemogenic endothelium prior to blood emergence.
Lie-A-Ling M, Marinopoulou E, Li Y, Patel R, Stefanska M, Bonifer C, Miller C, Kouskoff V, Lacaud G.
Blood. 2014 Sep 11;124(11):e11-20.

MOZ-mediated repression of p16(INK) (4) (a) is critical for the self-renewal of neural and hematopoietic stem cells.
Perez-Campo FM, Costa G, Lie-A-Ling M, Stifani S, Kouskoff V, Lacaud G.
Stem Cells. 2014 Jun;32(6):1591-601.

Smooth muscle cells largely develop independently of functional hemogenic endothelium.
Stefanska M, Costa G, Lie-A-Ling M, Kouskoff V, Lacaud G.
Stem Cell Res. 2014 Jan;12(1):222-32.

Systems Oncology

Oncogenic KRAS Regulates Tumor Cell Signaling via Stromal Reciprocation
Tape C, Ling S, Dimitriadi M, McMahon K, Worboys J, Leong HS, Norie I, Miller C, Poulogiannis G, Lauffenburger DL, Jørgensen C.
Cell (2016) 165(4). AOP Open Access

Reproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC.
Tape CJ, Worboys JD, Sinclair J, Gourlay R, Vogt J, Broster KM, Trost M, Lauffenburger DA, Lamont DJ, Jørgensen C.
Anal Chem. 2014 Sep 18. [Epub ahead of print]

Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome.
Worboys JD, Sinclair J, Yuan Y, Jørgensen C.
Nat Methods. 2014 Aug 24. doi: 10.1038/nmeth.3072. [Epub ahead of print]

PKA-regulated VASP phosphorylation promotes extrusion of transformed cells from the epithelium.
Anton KA, Sinclair J, Ohoka A, Kajita M, Ishikawa S, Benz PM, Renne T, Balda M, Jorgensen C, Matter K, Fujita Y.
J Cell Sci. 2014 Aug 15;127(Pt 16):3425-33.

Cell-specific Labeling Enzymes for Analysis of Cell-Cell Communication in Continuous Co-culture.
Tape CJ, Norrie IC, Worboys JD, Lim L, Lauffenburger DA, Jørgensen C.
Mol Cell Proteomics. 2014 Jul;13(7):1866-76.

Identification of hypoxia-regulated proteins using MALDI-mass spectrometry imaging combined with quantitative proteomics.
Djidja MC, Chang J, Hadjiprocopis A, Schmich F, Sinclair J, Mršnik M, Schoof EM, Barker HE, Linding R, Jørgensen C, Erler JT.
J Proteome Res. 2014 May 2;13(5):2297-313.

Transcriptional Networks in Lung Cancer

 

Role of microRNAs in maintaining cancer stem cells.
Garofalo M, Croce CM.
Adv Drug Deliv Rev. 2014 Nov 20. pii: S0169-409X(14)00277-4. doi: 10.1016/j.addr.2014.11.014. [Epub ahead of print] Review.

 

MicroRNAs as Anti-Cancer Therapy.
Garofalo M, Leva GD, Croce CM.
Curr Pharm Des. 2014 Jan 28. [Epub ahead of print]

Idiopathic pulmonary fibrosis is strongly associated with productive infection by herpesvirus saimiri.
Folcik VA, Garofalo M, Coleman J, Donegan JJ, Rabbani E, Suster S, Nuovo A, Magro CM, Di Leva G, Nuovo GJ.
Mod Pathol. 2014 Jun;27(6):851-62.