Techniques for Transgenic Mouse Production

Pronuclear Microinjection Platform

  1. Project design and discussion
  2. Generation of the targeting construct (optional)
  3. Injection of DNA into zygotes for production of founder mice
  4. Genotyping to identify F0 founders
  5. Mating of F0s to obtain germline-confirmed F1 mice

ES cell targeting Platform

  1. Project design and discussion
  2. Generation of targeting construct (optional)
  3. ES cell targeting and selection
  4. Screening and expansion of positive clones
  5. Microinjection of 2-3 positive clones into host blastocysts
  6. Chimera genotyping and breeding

CRISPR-Cas Platform

  1. Project strategy design and discussion
  2. Preparation of reagents based on specific project requirements
  3. Injection of CRISPR reagents into zygotes for production of founder mice
  4. Genotyping to identify F0 founders
  5. Mating of F0s to obtain germline-confirmed F1 mice