Biological Mass Spectrometry
The Biological Mass Spectrometry facility's remit is to support the protein and peptide mass spectrometry requirements of the CRUK Manchester Institute's research groups. This support covers a whole spectrum of activities ranging from the provision of routine services, implementation of new technologies onsite, through to dedicated collaborations designed to develop novel applications enabling cutting edge cancer research. One major role of the facility is to enable cancer researchers by developing workflows beneficial to their research projects. The facility currently boasts three complementary nano-LC-MS-MS systems, which form the backbone of both service and development portfolios.
We offer an array of qualitative and quantitative analyses in the areas of protein identification and post-translational modifications. These applications are currently supported with a combination of Thermo Orbitrap Lumos, Q Exactive HFx and Sciex 6600 mass spectrometers, each coupled to high resolution nano UPLC systems. We utilise high resolution nano LC as a key technology to enhance data quality using both conventional silica based and micropillar array chemistry based LC. We also offer offline multidimensional HPLC separations at the peptide level utilising both ion exchange and reverse phase chemistries to achieve maximum proteome penetrance.
The facility benefits from highly motivated and skilled staff with a combined 40 years of experience in the proteomics field.