High Content Screening

Since 2010 the instate has been utilising High Content Screening to numerate structural biology and response. Permitting the imaging and analysis of the phenotype of multiple cell populations so to study cellular responses, for applications such as drug discovery and 3D culture (spheroids and organoids).

• PerkinElmer Opera Phenix, a spinning disk based confocal screening system with a four dedicated sCMOS cameras, one each per detection channel. Magnification from 1.25-x60 (water). Automation allows loading of multi-well plates at room temperature or from an incubator. Designed for 3D/4D phenotypic screening
• PerkinElmer Operetta CLS, a fluorescence wide-field based illumination system for screening system multi-well plate
• Agilent Seahorse, The Seahorse XFe96 Analyser detects and measures discrete changes in cellular bioenergetics in real-time
• Columbus, analysis via a webpage interface to permit complex phenotypic analysis
• PerkinElmer Harmony 3D, for the analysis of organoids and spheroids
• Bitplane Imaris, visualisation and analysis of large data sets

3D organoid acquired on the Opera Phenix using plastic bottomed 96-well plate with the 20x water objective in confocal mode. The organoid is stained with DAPI in blue and a membrane marker in green.

3D nuclear detection using PerkinElmer Harmony is demonstrated.

Data from Christopher Below and Claus Jorgensen (Systems Oncology).