Post Translational Modifications
The Biological Mass Spectrometry facility offer an array of post-translational modification analyses including phosphorylation, acetylation, methylation, ubiquitination and SUMOylation.
We utilise a toolbox of different enzymes to enhance sequence coverage and enable more comprehensive PTM analyses appropriate to the experimental requirements.
GG Modified Ubiquitin peptide (K48)
Tandem Mass Spectra of an isopeptide carrying the Ub remnant GG
We have an extensive capability to enrich phosphopeptides using both Titania and iron-based chemistries to facilitate global phosphoproteomic profiling in combination with either metabolic or chemical labelling strategies.
We also employ Parallel Reaction Monitoring (PRM) to provide site specific quantitation with employment of Skyline and MS Stats.