In this study, the authors investigated the molecular mechanisms controlling the generation of HSCs during the intra-embryonic wave of haematopoiesis in the AGM and demonstrate a prominent role for the transcriptional repressors GFI1 and GFI1B in this process.

Here, the authors construct a comprehensive atlas of the endothelial-to-haematopoietic transition (EHT) continuum, as well as the subaortic niche cells in mouse embryonic aorta using a set of haemogenic endothelium (HE) reporter models.