MANIFEST (Multiomic ANalysis of Immunotherapy Features Evidencing Success and Toxicity) aims to build a robust platform of standardised biomarkers that will help predict response, resistance and toxicity and contribute to optimise the use of current and future immunotherapies.

In this study, the authors investigated the molecular mechanisms controlling the generation of HSCs during the intra-embryonic wave of haematopoiesis in the AGM and demonstrate a prominent role for the transcriptional repressors GFI1 and GFI1B in this process.

Here, the authors construct a comprehensive atlas of the endothelial-to-haematopoietic transition (EHT) continuum, as well as the subaortic niche cells in mouse embryonic aorta using a set of haemogenic endothelium (HE) reporter models.